| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
A novel proteomic approach identifies new interaction partners for proliferating cell nuclear antigen
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| Autores: |
Mesiet Cladiere, L
Norais, Cédric
Kuhn, Joelle
Briffotaux, Julien
Sloostra, Jerry W.
Ferrari, Elena
Hubscher, Ulrich
Flament, Didier
Myllykallio, Hannu
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| Data: |
2007-10
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| Ano: |
2007
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| Palavras-chave: |
Ribonuclease HII
Combinatorial peptide synthesis
PCNA
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| Resumo: |
During DNA replication and repair, many proteins bind to and dissociate in a highly specific and ordered manner from proliferating cell nuclear antigen (PCNA). We describe a combined approach of in silico searches at the genome level and combinatorial peptide synthesis to investigate the binding properties of hundreds of short PCNA-interacting peptides (PIP-peptides) to archaeal and eukaryal PCNAs. Biological relevance of our combined approach was demonstrated by identification an inactive complex of Pyrococcus abyssi ribonuclease HII with PCNA. Furthermore we show that PIP-peptides interact with PCNA largely in a sequence independent manner. Our experimental approach also identified many so far unidentified PCNA interacting peptides in a number of human proteins. (c) 2007 Elsevier Ltd. All rights reserved.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/2007/publication-3297.pdf
DOI:10.1016/j.jmb.2007.06.056
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| Editor: |
Elsevier
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| Relação: |
http://archimer.ifremer.fr/doc/00000/3297/
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| Formato: |
application/pdf
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| Fonte: |
Journal of Molecular Biology (0022-2836) (Elsevier), 2007-10 , Vol. 372 , N. 5 , P. 1137-1148
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| Direitos: |
2007 Elsevier Ltd All rights reserved
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